The study is where Alexander performed anatomical studies and experiments on animals to extract vitae. This is observed in a flashback when Alexander is heard sawing open a dog. In the end, he gave up his study on animals to focus on humans, as dogs do not produce sufficient amounts of vitae. Occasionally, you will hear the miserable barking of a dog. On low sanity, the portrait of Alexander will appear horribly disfigured.As Daniel walks down the corridor, the Shadow will roar and the resulting tremors will fracture one of the windows, allowing access to an isolated room when broken by Daniel.
The cycle flow control rod for the Machine Room is kept in the study. Unfortunately, a cave-in blocked the room with the rod, so Daniel must break the weakened window on the far left and hop along the edge of the building to get there. The Shadow, aware of him, will immediately overtake the nearby forest and consume the area, felling pine trees and frightening Daniel. In this remote section of the Study, one of Alexander's memory capsules can be found.
In an earlier version of the game, there was also a phonograph like the ones in Amnesia: Justine, containing a recording of a woman accusing Daniel of killing her, but also offering him redemption in exchange for answers. As of version 1.2 with Justine completed, the phonograph isn't present.
- Whenever Daniel is near or in the dissecting room, he can faintly hear the sounds of dogs whimpering or crying. These sounds are residual hauntings of the dogs that were killed by Alexander during his experiments in search of vitae.